TWISTING AND SWIVELING DOMAIN MOTIONS IN CAS9 TO RECOGNIZE TARGET DNA DUPLEXES, MAKE DOUBLE-STRAND BREAKS, AND RELEASE CLEAVED DUPLEXES

Twisting and swiveling domain motions in Cas9 to recognize target DNA duplexes, make double-strand breaks, and release cleaved duplexes

The CRISPR-associated protein 9 (Cas9) has been engineered as a precise gene editing tool to make double-strand breaks.CRISPR-associated protein 9 binds the folded guide RNA (gRNA) that serves as a binding scaffold to guide it to the target DNA duplex via a RecA-like strand-displacement mechanism but without ATP binding or hydrolysis.The target sea

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THE SIGNIFICANCE OF WAQF FOR ECONOMIC DEVELOPMENT

Terdapat anggapan dari sejumlah ilmuan Barat yang menyatakan bahwa institusi wakaf tidak lagi memainkan fungsi ekonomi yang signifikan dalam konteks perekonomian modern sebagaimana pada masa-masa sebelumnya.Menurut mereka, wakaf pada masa kini justru muncul sebagai faktor yang menghambat pembangunan di negara-negara Muslim.Artikel ini dimaksudkan u

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The institutionalisation of populist political discourse and conservative uncivil society in the European Union

This article analyses the ideational features of conservative civil society groups at EU level and compares them to progressive groups.Through a frame analysis of the textual materials of these two types of organisations, I examine their reactions to the success of populist formations in several European member states and at EU level.I argue that t

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